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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
18/05/2007 |
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Data da última atualização: |
08/06/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZUCCHI, T. D.; ZUCCHI, F. D.; POLI, P.; MELO, I. S. de; ZUCCHI, T. M. A. D. |
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Afiliação: |
Tiago Domingues Zucchi, ICB-USP; Fernando Domingues Zucchi, ICB-USP; Paola Poli, Dipartimento di Genetica Antropologia Evoluzione, Universita di Parma; ITAMAR SOARES DE MELO, CNPMA; Tania M. A. D. Zucchi, ICB-USP. |
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Título: |
A short-term test adapted to detect the genotoxic effects of environmental volatile pollutants (benzene fumes) using the filamentous fungus Aspergillus nidulans. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Journal of Environmental Monitoring, Washington, DC, v. 7, n. 6, p. 598-602, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
With the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the genotoxic/carcinogenic harmful side effects widely connected to benzene. This is the first description of a sensitive, rapid and inexpensive test able to detect the submutagenic dose effects of volatile environmental compounds. In addition, despite concentrating on benzene the same test can be applied to many other pollutants, volatile or not. Additionally, the test can also be used to detect the antigenotoxic properties of foods and drugs. MenosWith the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the geno... Mostrar Tudo |
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Palavras-Chave: |
Microbiologia ambiental. |
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Thesagro: |
Aspergillus Nidulans; Fungo; Microbiologia; Poluente; Poluição ambiental. |
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Thesaurus Nal: |
Carcinogens. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02770naa a2200253 a 4500
001 1015437
005 2015-06-08
008 2005 bl uuuu u00u1 u #d
100 1 $aZUCCHI, T. D.
245 $aA short-term test adapted to detect the genotoxic effects of environmental volatile pollutants (benzene fumes) using the filamentous fungus Aspergillus nidulans.$h[electronic resource]
260 $c2005
520 $aWith the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the genotoxic/carcinogenic harmful side effects widely connected to benzene. This is the first description of a sensitive, rapid and inexpensive test able to detect the submutagenic dose effects of volatile environmental compounds. In addition, despite concentrating on benzene the same test can be applied to many other pollutants, volatile or not. Additionally, the test can also be used to detect the antigenotoxic properties of foods and drugs.
650 $aCarcinogens
650 $aAspergillus Nidulans
650 $aFungo
650 $aMicrobiologia
650 $aPoluente
650 $aPoluição ambiental
653 $aMicrobiologia ambiental
700 1 $aZUCCHI, F. D.
700 1 $aPOLI, P.
700 1 $aMELO, I. S. de
700 1 $aZUCCHI, T. M. A. D.
773 $tJournal of Environmental Monitoring, Washington, DC$gv. 7, n. 6, p. 598-602, 2005.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Solos. |
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Data corrente: |
08/02/2022 |
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Data da última atualização: |
15/02/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LEITE, F. F. G. D.; ALVES, B. J. R.; NÓBREGA, G. N.; CORDEIRO, R. C.; CESÁRIO, F. V.; SILVEIRA, J. G. da; ARMACOLO, N. M.; RODRIGUES, R. de A. R. |
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Afiliação: |
FERNANDA FIGUEIREDO GRANJA DORILÊO LEITE, UFF; BRUNO JOSE RODRIGUES ALVES, CNPAB; GABRIEL NUTO NÓBREGA, UFF; RENATO CAMPELLO CORDEIRO, UFF; FERNANDO VIEIRA CESÁRIO, IABS; JÚLIA GRAZIELA DA SILVEIRA, UFV; NATASSIA MAGALHÃES ARMACOLO, UEL; RENATO DE ARAGAO RIBEIRO RODRIGUES, CNPS. |
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Título: |
Greenhouse gases (GHG) quantification with static chambers in Latin America: is it improving through time? |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. WCCLF 2021 proceedings. Brasília, DF: Embrapa, 2021. p. 79-84. WCCLF 2021. Evento online. |
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Idioma: |
Inglês |
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Conteúdo: |
Manual static chamber is one of the most widespread methods for the quantification of greenhouse gases (GHGs) emission from agricultural systems. But there are concerns about the reliability of measurements and a necessity to assess the static chambers used in the studies performed in the countries of Latin America. Thus, this study aims to investigate the quality of static chambers used for GHGs measurement in scientific articles following international recommendations and assess the confidence level of static chambers. A systematic review of databases was conducted to identify peer-reviewed articles that used the static chamber method in Latin América. A total of 90 articles were analyzed and separated according to the publication year (2000- 2008, 2009-2015, 2016-2020). This time interval was selected to assess the influence of key publications with guidelines and criteria for the method published in 2008 and 2015. Six design and deployment chamber characteristics were evaluated. The chambers received a global score for a confidence level according to their characteristics scores and the weight of each one. The percentage of articles with high confidence level increased within the time, and the number of articles with low confidence level reduced. Researchers should continue to follow the Global Research Alliance protocols, especially the most updated ones. |
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Thesaurus NAL: |
Greenhouse gas emissions; Methodology; Nitrous oxide. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/231332/1/GREENHOUSE-GASES-GHG-QUANTIFICATION-WITH-STATIC-CHAMBERS-IN.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/231055/1/Greenhouse-gases-GHG-quantification-with-static-chambers-2021.pdf
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Marc: |
LEADER 02225nam a2200229 a 4500
001 2139892
005 2022-02-15
008 2021 bl uuuu u00u1 u #d
100 1 $aLEITE, F. F. G. D.
245 $aGreenhouse gases (GHG) quantification with static chambers in Latin America$bis it improving through time?$h[electronic resource]
260 $aIn: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. WCCLF 2021 proceedings. Brasília, DF: Embrapa, 2021. p. 79-84. WCCLF 2021. Evento online.$c2021
520 $aManual static chamber is one of the most widespread methods for the quantification of greenhouse gases (GHGs) emission from agricultural systems. But there are concerns about the reliability of measurements and a necessity to assess the static chambers used in the studies performed in the countries of Latin America. Thus, this study aims to investigate the quality of static chambers used for GHGs measurement in scientific articles following international recommendations and assess the confidence level of static chambers. A systematic review of databases was conducted to identify peer-reviewed articles that used the static chamber method in Latin América. A total of 90 articles were analyzed and separated according to the publication year (2000- 2008, 2009-2015, 2016-2020). This time interval was selected to assess the influence of key publications with guidelines and criteria for the method published in 2008 and 2015. Six design and deployment chamber characteristics were evaluated. The chambers received a global score for a confidence level according to their characteristics scores and the weight of each one. The percentage of articles with high confidence level increased within the time, and the number of articles with low confidence level reduced. Researchers should continue to follow the Global Research Alliance protocols, especially the most updated ones.
650 $aGreenhouse gas emissions
650 $aMethodology
650 $aNitrous oxide
700 1 $aALVES, B. J. R.
700 1 $aNÓBREGA, G. N.
700 1 $aCORDEIRO, R. C.
700 1 $aCESÁRIO, F. V.
700 1 $aSILVEIRA, J. G. da
700 1 $aARMACOLO, N. M.
700 1 $aRODRIGUES, R. de A. R.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Agroindústria de Alimentos. | |
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Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Suínos e Aves. | |
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Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Amazônia Oriental. | |
| Registros recuperados : 925 | |
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